1.Objective: To verify Mulberry leaf extract’s anti-viral properties, this clinical application validation experiment was specially carried out on a group of laying hens with suspected virus infection.
2. Materials: Mulberry leaf extract (DNJ content 0.5%), provided by Hunan Geneham Pharmaceutical Co., Ltd.
3. Site: In Guangdong XXX Agricultural Technology Co., Ltd. (Chicken house: G23, Batch: G1901, Day-old: 605-615) from 1 to 10 September, 2020.
4. Methods: 50,000 suspected virus infected laying hens were chose in a 10days feeding trial with addition of DNJ (0.5%) 1kg/ton feeding, to observe and record the production performance indexes of laying hens. The feeding management as per routine management of the chicken house and no other drugs were added during this experiment.
5. Results: see Table 1
Table 1 The improvement of dietary Mulberry leaf extract on productivity in Laying hens
|Production Phase||Average laying rate
|Unqualified egg rate
|Average egg weight, g / egg||The average mortality number
|10 days before the experiment||78.0||51%||63.4||65|
|10 days during the experiment||80.2||43.5%||63.0||23|
|One week after the experiment||81.3||42.4%||63.4||12|
Table 1 results show that:
5.1 Laboratory diagnosis proves the hens were avian influenze H9 infected, and the mortality number is 65 hens/day before treatment (early phase), 23 hens/day during the treatment (Middle phase), 12 hens/day after treatment, which proves that Mulberry leaf extract provides benefit to influenza virus (H9 subtype) inhibitory and the laying hens with increased livability.
Suggestion: The cooperatively use of antipyretic (Bupleurum distilled liquid) during the pathogenic period can maintain the livability rate. In case of mixed bacterial infection, such as Haemophilus paragallinarum, mycoplasma, Escherichia coli, Salmonella, Staphylococcus, Clostridium perfringens etc., comprehensive treatment with effective drugs (such as flos magnoliaepowder, Yin-huang extract, Yanlikang, lysozyme, probiotics etc.) is needed.
5.2 Mulberry leaf extract help to effectively stop the laying rate dropping caused by virus infection. An 1.8% increased laying rate during the 10days treatment and an 1.1% increased rate again one week after mulberry leaf extract withdrawal had observed.
5.3 The experiment also shows a reduced feed intake due to high dosage and a slightly affected egg weight (minimum egg weight 62.7g). These adverse reactions are reversible and will be returned to normal level after withdrawal.
Remedial measures: Add water-type plant essential oil that can improve feed intake on the 5th day after mulberry leaf extract withdrawal and reduce the impact on feed intake.
Suggestion: Lower the dosage of mulberry leaf extract. The subsequent clinical application shows that the dosage can be adjusted to 200g /ton feeding. Higher dosage can be used for 3 days if needed, and then adjust to the normal dosage. Probiotics and plant essential oil should be used cooperatively to reduce the inhibitory effect on feed intake.
5.4 Mulberry leaf extract can effectively reduce the number of unqualified eggs caused by virus infection. The rate of unqualified eggs is 51%before treatment, 43.5% during treatment and 42.4% after treatment.
5.5 Laboratory diagnosis proves the hens were avian influenze H9 infected, no other therapeutic schedule can effectively keep the livability rate in the early phase, but mulberry leaf extract is effective for the disease.
It can thus be concluded that: mulberry leaf extract is effective for keeping the laying hens away from viral diseases , and promote the livability rate,promote the productivity and maintain qualified egg rate; mulberry leaf extract has significant effect of clinically treat viral diseases, it is worth to be applied widely.
Post time: Dec-01-2020